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產品名稱:SM10λpir菌株(ZK230)

貨號 規格 價格 訂購數量 是否現貨
ZK230 0.3ml/支 580 - + 有貨

■ 產品介紹

Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereo.E. coli SM10 λpir strain contains the pir gene (lysogenized with λpir phage) and it is designed for cloning and propagation of plasmids with R6K origin of replication sequence (such as pUT mini-Tn5 vectors). The plasmids containing the R6K origin of replication require the R6K-spicific replication protein lpir gene for propagation.

基因型為: thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu


■ 基本信息

抗性:Kan

培養基:LB

菌株類別:大腸桿菌

培養條件:37℃,有氧,LB

質粒轉化:42℃熱激

保存方式:15%甘油,-80℃

基本應用:用于基因克隆


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